The purpose of the study was to examine the barriers to health service delivery in Juba teaching Hospital in Central Equatorial, South Sudan. The objectives of the study included: to examine the effect of Human Resources for Health on health service delivery at Juba Hospital, to assess the effect of Health Infrastructure on health service delivery at Juba teaching Hospital and to establish the extent to which Healthcare Consumable affect the health service delivery in Juba civil teaching Hospital. The study used cross-sectional survey research design adopting both quantitative and qualitative approaches. The researcher used a sample of 80 respondents. Data was collected using questionnaires and interview guides. Findings from the study revealed that there is a strong positive relationship between human resources for health and health service delivery. There was a strong positive relationship between health infrastructure and health service delivery. There was a very strong positive relationship between health care consumables and health service delivery. The finding therefore revealed that adequate human resources, functioning health infrastructure and availability of healthcare consumables is a necessity if health service delivery is to be achieved in Juba teaching Hospital. Health service delivery should be prioritized by government and should be given more attention and increase its national budget allocation. It is recommended that ministry of health should recruit adequate staff and deploy them evenly in the various health departments, good health infrastructure is related to better health service delivery, and government should consider constructing adequate accommodation for medical staff as priority and adequate health care consumables is related to better health service delivery. The government of South Sudan should strengthen the national supply chain for essential health commodities to ensure that South Sudanese have access to essential drug and general services.

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